First Live-Cell Imaging Experiments Completed

We are thrilled to share that the CytoArchiLab has successfully completed its first live-cell imaging experiments. Using human RPE1 cells, we visualised two complementary aspects of cytoskeletal dynamics in living cells.

We gratefully acknowledge Manuel Théry and Louise Bonnemay for kindly providing the cell lines used in these experiments.

Lifeact-GFP — Actin dynamics

Lifeact-GFP labels filamentous actin, allowing us to follow the remodelling of the actin cortex and protrusive structures in real time.

Human RPE1 cell expressing Lifeact-GFP. Imaged on a Zeiss LSM 900 (Fast Airyscan mode) at the PPDH Central Research Lab, Faculty of Dentistry, HKU.

EB3-GFP — Microtubule plus-end dynamics

EB3-GFP marks growing microtubule plus-ends, appearing as comet-like signals that reveal the polarity and dynamics of the microtubule network.

Human RPE1 cell expressing EB3-GFP. Imaged on a Zeiss LSM 900 (Fast Airyscan mode) at the PPDH Central Research Lab, Faculty of Dentistry, HKU.

These first recordings establish our live-cell imaging pipeline as the lab ramps up its experimental work on cytoskeletal organisation and mechanics in culturing cell lines.